ABSTRACT
Objective: To optimize the inclusion process for the volatile oil in Huaweishu Granules. Methods: Taking the ratio of volatile oil to β-cyclodextrin, inclusion temperature, stirring speed and stirring time as the factors, the inclusion process conditions were optimized by orthogonal test, the weight coefficients of each index were determined by entropy weight method, and the inclusion complex was verified by thin layer chromatography. Results: The inclusion rate of volatile oil obtained by stirring method was the highest, with the ratio of β-cyclodextrin to volatile oil of 10:1, the inclusion temperature was 40 ℃, the stirring time was 1 h, and the stirring rate was 400 r/min. Conclusion: The optimized volatile oil inclusion process is stable and feasible, with high inclusion rate of volatile oil and yield of inclusion compound.
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Objective: To optimize the integration technology on field processing and processing crude drugs of Ligusticum chuanxiong (LC) and provide the scientific evidence for the integration of LC. Methods: Single factor test and orthogonal method was used to investigate the three significant influencing factors, which were water content, drying temperature, and slice thickness. Based on the appearance character and active component (ferulic acid, volatile oil, and extract) of LC, the integrated process was selected by comprehensive scoring method. To investigate the stability of the process, three batches of integrated slices were processed according to the optimized integrated process; And three batches of corresponding traditional slices were processed according to the method stipulated in Chinese Pharmacopoeia (2015 edition), and the quality of the two kinds of slices was compared. The rat migraine model was induced by nitroglycerin. The effects of two different processed slices on migraine were compared by recording the times of head flick and head scratching, the level of NO in serum and the activity of NOS in serum of rats. Results: The optimum integrated process was as follows: drying fresh LC until water content was 28%, cutting 2 mm thick slices, drying at 50 ℃ in drying box (about 6 h). The integrated process had good stability and repeatability, and the quality of integrated slices was close to that of traditional slices. Compared with the model group, two processed slices could significantly reduce the number of head flick and head scratch (P < 0.01, 0.001), and decreased the activity of NOS (P < 0.01), and the level of NO decreased significantly (P < 0.01). The pharmacodynamics of the two process pieces was similar. Conclusion: The integrated processing and traditional processing of Chuanxiong Rhizoma decoction has good similarity in character, effective component content and treatment of migraine. Moreover, the integrated processing has standardized the production of Chuanxiong Rhizoma decoction slices from the source. Under the premise of ensuring the quality of Chinese herbal slices, the production cycle is shortened and the production cost is saved. It is a kind of traditional Chinese medicine slices production mode which is worth popularizing.
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Objective: To optimize a technology of integration on field processing and processing crude drugs of Polygoni Multiflori Radix (PMR) and to provide the scientific evidence for the integration of PMR. Methods: Orthogonal test was used to optimize the integration technology on primary processing and reprocessing with two major characteristic components (stilbene glucoside and combined anthraquinone). To compare the differences between the integration technology and traditional technology on pharmacological activity by models of constipation and swelling of ear in mice with Various index (small intestinal propulsion rate, first defecation time, fecal water content, swelling degree of ear, and inflammatory factors). Results: The results showed that the integration technology of 50℃, 16 h was better, and there was significant difference in chemical composition and Laxative effect between the integration technology and traditional technology. The anti-inflammatory effect of the integrated process was better than that of the traditional technology. Conclusion: The technology of integration of field processing and processing crude drugs is feasible and the operation is good.
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This study was aimed to screen out a safe and effective traditional Chinese medicine (TCM) compound prescription in insomnia treatment, through the method of uniform design and comprehensive scoring method. It provided candidate drugs to further research and development of TCM new drug. U7(76) uniform design was used to divide groups. The test was given on sleep time prolonging effect of mice injected with pentobarbital sodium. The subliminal dosage of pentobarbital hypnosis was tested on mice injected with pentobarbital sodium. The acute toxicity (LD50) was tested on mice. Comprehensive scoring method was applied to evaluate the effect to determine the optimum dose. The results showed the optimized prescription was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The optimum ratio was 1:3. It was concluded that the uniform design was applied in the experimental design with 3 factors and 6 levels. Comprehensive scoring method was used to evaluate the combined effect. The optimized prescription that put benefit and risk into consideration was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The best ratio was 1:3, which can be further developed.
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AIM: To optimize the random amplified polymorphic DNA-Polymerase chain reaction(RAPD-PCR) system of Bupleurum chinense DC by comprehensive scoring method. METHODS: The L_(16)(4~5) orthogonal diagram was applied to studying the effects of different concentrations of Taq polymerase,Mg~(2+),dNTPs,primer and DNA on Bupleurum chinense DC.RAPD-PCR results and to get a suitable PCR system. RESULTS: A suitable RAPD reaction system was developed,i.e.1?Taq polymerase reaction buffer,2.5 mmol/L Mg~(2+),1.5 U Taq DNA polymerase,0.15 mmol/L dNTPs,0.4 ?mol/L primer and 25 ng genomic DNA templates in total 25 ?L reaction solution. CONCLUSION: The results show that the comprehensive scoring method could be used successfully to optimize the RAPD-PCR system.